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Regarding suitability of Eppendorf bioprocess equipment in GMP-regulated applications, please reach out to your Eppendorf sales representative.

Yeast Fermentation

Bioreactor control systems for yeast fermentation

Advantages of yeast fermentation in the biopharmaceutical industry

What is yeast fermentation? When thinking of yeast fermentation in bioprocessing, food, ethanol and biofuel production may come to mind. However, yeast is a versatile microorganism that is also widely used in the biopharmaceutical industry, for the expression of recombinant proteins to prevent or treat diseases. Industry-relevant yeast species include Saccharomyces cerevisiae and Pichia pastoris.
Yeast fermentation is well suited for the large-scale production of therapeutic proteins, due to:
  • Fast growth rate
  • Easy genetic manipulation
  • High cell productivity
  • Ability to be grown at high cell densities
  • High tolerance to deviations in pH and temperature (compared to mammalian cells)
  • Cheaper costs and shorter cultivation cycles than mammalian cell cultures

Microorganisms including and yeast are ideal for large-scale bioprocessing due to their high yields and fast growth. However, an advantage of yeast cells in particular is that they are eukaryotic and so they are more comparable to human cells. Consequently, yeast cells can perform a range of post-translational protein modifications that bacteria are unable to. These modifications are essential for the functioning of therapeutic proteins and include glycosylation, protein folding and phosphorylation.

What properties make a bioreactor system suitable for microbial production?

Find out, which bioreactor features can support achieving high cell densities, simplify scale-up, and streamline bioprocess optimization.

Pichia pastoris fermentation: Basic information

Pichia pastoris is a popular species for yeast fermentation in the large-scale production of recombinant proteins. This yeast species is capable of achieving high cell densities and protein yields, and is also easy to scale up, demonstrating high levels of reproducibility in small-scale, through to bench-scale and production-scale bioprocesses. In shakers or bioreactors of any size, Pichia pastoris can be used as a powerful protein expression system.

Optimizing protein expression in yeast fermentation

Developing a commercially viable yeast fermentation process requires the optimization product yields. An adequate supply of nutrients must be ensured, to support high cell densities. The promoter system is another important factor that determines protein expression. Here we will have a look at some factors that can contribute to the optimization of protein expression in yeast.

Methanol-free induction of protein expression in Pichia pastoris

For protein expression in Pichia pastoris, traditionally, the AOX1 promoter is used that requires the presence of methanol for its activation. Concerns of methanol have been raised due to its toxicity and flammability, resulting in a need to move away from the use of methanol in yeast fermentation.To overcome this, other Pichia pastoris promoters such as PUPP can function in the presence of other carbon sources including glycercol. These promoters have enabled the development of safer methanol-free Pichia pastoris expression systems. Learn how to set up methanol-free Pichia pastoris bioprocessing systems!

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Choosing between batch, fed-batch, and continuous fermentation

The operation mode of a yeast culture relates to the strategy of feed addition, and is an important consideration for yields and productivity. Read more how choosing between fatch fermentation, fed-batch fermentation and continuous culture can be used to optimize your bioprocess.

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Automate culture feeding - How can this improve yeast fermentation?

Although substrate can be added manually, automated feeding can provide several benefits to your bioprocess. For instance, automated feed systems continuously monitor substrate concentration in the yeast culture to allow for more precise feed timing and dosage calculation. Automated feeding ensures that substrate concentrations remain optimal in the culture to maintain a steady culture environment, and maintain maximum cell yields and product quality. Feed automation also saves on labor and time, removing the need for manual substrate monitoring and addition.

Bioreactor systems for yeast fermentation from 60 mL to 2,400 L

Eppendorf bioreactor systems demonstrate robust and scalable fermentation across several applications, including the use of Pichia pastoris cultures for biologics production.

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